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. 2016 Dec 21;11:297–311. doi: 10.1016/j.redox.2016.12.022

Fig. 8.

Fig. 8.

Restorative effect of mitoQ on mitophagy, ROS production and apoptosis via Nrf2 and PINK. A: Confocal microscopic image showing that mitoQ ameliorated intracellular ROS production, mitochondrial ROS production and apoptosis in HK-2 cells subjected to HG exposure, as demonstrated by H2-DCFDA, MitoSOX and TUNEL assays, respectively. These effects were partially blocked by Nrf2 siRNA or PINK-siRNA. Transfection with Nrf2 siRNA or PINK-siRNA also attenuated LC3 and mitochondria colocalization intensity in cells treated with mitoQ under HG conditions. B–D: Bar graphs represent intracellular and mitochondrial ROS and apoptosis levels, as demonstrated using H2-DCFDA, MitoSOX and TUNEL assays. E: The effect of Nrf2 siRNA or PINK-siRNA on LC3II, p62, Drp1, and TOM20 expression in HK-2 cells subjected to HG exposure with or without mitoQ using immunoblot assays. E1-E5: Quantification of average Western blot band intensities. *P<0.05 compared to LG; # P<0.05 compared to HG. $P<0.05 compared to HG+mitoQ.