Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 23;7:14021. doi: 10.1038/ncomms14021

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a–c) Northern blot analysis of PTC-containing PGK1 reporter mRNA. (a) upf1Δ cells complemented with either ATPase-deficient UPF1 (UPF1-DE) or the same mutant also lacking RNA-binding activity (UPF1-DE/RR). (b) PGK1-PTC344 reporter mRNA in upf1Δ cells expressing wild-type (WT) or mutant UPF1 (DE) and also lacking UPF2 (upf2Δ), UPF3 (upf3Δ) or both (upf2Δupf3Δ). (c) PGK1-PTC344 reporter mRNA in upf1Δ cells expressing ATPase-deficient UPF1 (DE), mutant UPF1 relieved for allosteric inhibition of ATPase and helicase activities (F), or UPF1 harbouring both mutations (F/DE) in cells either with or without UPF2 (upf2Δ). Full-length reporter mRNA (FL) and 3′ RNA fragments (arrow) are indicated. RNA levels were normalized to NMD-insensitive SCR1 RNA. Results are representative of three independent experiments. Nt, nucleotide.