Fig. 3.

Panel A. Motility of exsheathed third-stage larvae (xL3s) of Haemonchus contortus incubated in culture medium (LB* + 1% DMSO) (untreated), and in the presence of 100 μM of either tolfenpyrad (‘hit’ compound) or moxidectin (control) at three distinct time points; at each data point, motility was normalised to time (0 min). Panel B. Based on results in panel A, oxygen consumption was measured seven times (8 min each) over a period of 1 h in untreated xL3s, and xL3s treated with the same concentration (100 μM) of tolfenpyrad (‘hit’ compound) or moxidectin (control) using a flux analyser. The mean values from 10 replicates (motility) and 8 replicates (oxygen consumption) from two separate experiments are shown, with variation between measurements displayed as a standard error of the mean (SEM). *^/** indicate values (mean ± SEM) which are significantly different from one another (P < 0.05 and P < 0.01, respectively), determined using a two-way ANOVA and Dunnett's multiple comparison test.