Fig. 1.

Families of TRP channel genes in S. mansoni. Maximum likelihood tree of predicted S. mansoni TRP channel protein sequences, shown with closest human TRP channel subtype (color coded by subfamily). Note the absence of predicted TRPV-like sequences. Relative expression levels (+, ++, +++, ++++) at different parasite stages are derived from transcriptomics data (Protasio et al., 2012) at http://www.genedb.org/Homepage/Smansoni as well as our own analysis using RT-PCR against S. mansoni RNA. C, cercariae; S-3, 3-h schistosomula; S-24, 24-h schistosomula; A, adults. Tree was derived using alignment and tree building software as implemented in MEGA6 (Tamura et al., 2013). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)