Fig. 3.

Bromocriptine effects a long lasting impairment of planarian movement. (A) D. japonica mobility before (pre) or at various time periods after a 20 min exposure (post drug, spanning time = ‘0’ to 1 week) to the S7.1R antagonists bromocriptine (BRM, 5 μM) and cyproheptadine (CYPH, 50 μM). (B&C) Quantification of images shown in (A) before and after drug washout. Each datapoint represents the mobility of a single worm (3 petri dishes, 10 worms in each dish). Plotted line connects the population mean for each condition. (D) Minimum intensity projections show movement profiles for worms treated with no drug (top), mianserin (middle; 10 μM, 20 min) and PZQ (bottom; 75 μM, 15 min), before drug exposure (left), during drug exposure (middle) and 2hrs after drug removal (right). (E) Effects of mianserin (10 μM) and praziquantel (100 μM) on 5-HT (1 μM) evoked cAMP signaling through the S7.1R. PZQ, unlike mianserin, did not block 5-HT action at the planarian S7.1R. Data are representative of n ≥ 3 assays, and represent mean ± s.e.m. P value, * = p < 0.05.