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CLSM microimages (A) and FCM results (B) of RAW 264.7 cells incubated with FITC-labled DS-g-MTX or Dex-g-MTX for 2 h after activited by LPS (+) or not (-). Cells of the control group were incubated with PBS. Scale bars: 10.0 μm. Cytotoxicity of DS-g-MTX, Dex-g-MTX, and free MTX after coincubation for 72 h toward RAW 264.7 cells activated by LPS in vitro (C). Data were presented as mean ± standard deviation (SD; n = 3).
