Figure 2.

In vitro characterization of HA binding to LYVE-1. (A) LYVE-1 and bovine serum albumin of different concentrations were coated on 96-well plates and Cy5.5-HA10K was added for incubation. After washing, the plates were visualized by fluorescence images. (B) Quantification of Cy5.5-HA binding to LYVE-1 proteins and BSA. (C) At 2 h after Cy5.5-HA10K injection, popliteal LNs were harvested and sectioned into 10 μm slices. Fixed slices were then stained against LYVE-1. Co-localization of LYVE-1 (red) and HA (green) was observed. Nuclei were stained as blue with DAPI. The scale bar is 20 μm.