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. 2017 Jan 1;7(1):153–163. doi: 10.7150/thno.17085

Figure 6.

Figure 6

Mapping UM-SCC-22B tumor metastasis in sentinel lymph nodes by dual-tracer fluorescence optical imaging. (A) LN metastasis model was developed by hock injection of Fluc+ UM-SCC-22B cells and the metastases were confirmed by BLI. The original tumor sites were pointed by arrows and tumor metastasized LNs by arrow heads. (B) IRDye800-cetuximab (anti-EGFR antibody) was injected intravenously at 24 h before imaging and Cy5.5-HA10K was administered locally at 1 h before imaging with a Maestro II optical imaging system. The fluorescence signal was unmixed based on the corresponding specific spectra from Cy5.5 (designated as blue) and IRDye800 (designated as red). The skin was peeled to mimic the operative setting. (C) Bright field and (D) optical imaging of excised LNs. LN without tumor invasion showed red color only from Cy5.5-HA. Partially tumor invaded LN showed mixed signals from both Cy5.5-HA10K and IRDye800-cetuximab. Fully tumor occupied LN showed signal only from IRDye800-cetuximab since the lymphatic vessels were blocked by tumor tissue. (E) H&E staining of corresponding LNs. T, tumor tissue; LN, lymphatic tissue.