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. 2017 Jan 1;7(1):180–195. doi: 10.7150/thno.17133

Figure 5.

Figure 5

miR-140-5p maintains the function of articular chondrocytes (A) Normalized gene expression levels in SMSC-Exos detected by miRNA microarray. Three independent samples were used. (B) Gene expression levels of highly-expressed genes in SMSC-Exos and gene expression levels of miR-140-5p and miR-140-3p. (C) Efficiency of shRalA #1, shRalA #2 and RalA lentiviral vectors were detected by qPCR. This experiment was repeated three times. *P < 0.05 compared to chondrocytes transfected with empty vector. (D) Aggrecan or SOX9 gene expression levels of chondrocytes transfected with miR-140-5p, RalA or shRalA #1. This experiment was repeated three times. *P < 0.05 compared to chondrocytes transfected with empty vector. (E) Protein expression levels of Col II, Aggrecan, SOX9 and RalA were analysed using western blotting. The results of statistical analysis of three independent replicates are also shown. *P < 0.05 compared to Empty Vector. #P < 0.05 compared between each other. (F) Expression level changes of miR-140-5p in SMSC-Exos, SMSC-140-Exos, or in articular chondrocytes which were treated with SMSC-Exos (AC-SMSC-Exos) or SMSC-140-Exos (AC-SMSC-140-Exos). This experiment was repeated three times.