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The resected prostate was embedded in agar in a localizer with a 4 mm marker profile and ex-vivo CT was performed by using reference points on the localizer for orientation (A). In a cutting device 4 mm step sections were cut (B). Whole-mount sections and ex-vivo CT images were merged under the guidance of markers on the localizer (C). Ex-vivo and in-vivo prostate were registered manually by two specialists in consensus (D).
