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Cell morphology of isolated cell fractions. Liver cells were isolated from the livers of FVB/N mice by a collagenase perfusion method. Hepatocyte (Hep) fraction was purified by Percoll isodensity centrifugation, and liver sinusoidal endothelial cell (LSEC) fraction was condensed by magnetic cell sorting. Isolated cells were seeded on type I collagen-coated six-well culture dishes at a density of 7.5 × 105 cells per well and were cultured for 48 h. Scale bars: 100 μm.
