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. 2012 May 8;3(1-3):89–95. doi: 10.3727/215517912X639405

Figure 3.

Figure 3

Evaluation of the iPS cells cryopreserved with Cell Banker 3 after 3 and 12 months of storage. (A) The viability of the cryopreserved iPS cells after 3 and 12 months of storage at −80°C. 1, After 3 months; 2, after 12 months. (B) The proliferation of cryopreserved iPS cells after 3 and 12 months of storage. 1, After 3 months; 2, after 12 months; 3, only the MEF feeder cell layer. The data are the means and SD of three independent experiments. (C) The phase-contrast photomicrographs of (1) cryopreserved iPS cells after 12 months. The fluorescent photomicrograph (2) exhibited the expression of green fluorescent protein derived from undifferentiated iPS cells. (C) Scale bar: 100 μm.