Figure 2.

The cross-talk of bacterial pathogens with neutrophil effector proteins. Two groups of subjects were investigated, including the UTI group and the EXF (exfoliation of urothelial/squamous epithelial cells) group. The neutrophil granule proteome subset was quantified for UP and SU fractions and is presented in an interactive chart with bacterial proteins likely expressed in response to host immune defenses. Based on the comparisons shown in Supplemental Figure S5, 103 granule proteins identified from SU and UP fractions were selected for this graphic. The iBAQ intensities were summed, divided by the iBAQ intensities of entire proteome in each case and then displayed in the histogram. Calculations were performed for UP (blue) and SU (red) fractions. The average neutrophil granule protein content is 28.4% (± 14.1, SD; n=21) and 7.5% (± 5.4) in UP and SU samples, respectively. From the UP datasets only, iron and zinc acquisition proteins were summed based on PSMs and divided by all bacterial PSMs. Oxidative stress response proteins inactivating reactive oxygen and nitrogen species (ROS and RNS, respectively) were summed and also divided by all bacterial PSMs. The plot shows a trend of high neutrophil activities in samples representing UTIs that correlated well with the expression of iron acquisition and ROS/NOS response proteins.