Figure 5.

Specificity of ⁶⁸Ga-NOTA-MSA uptake in myocarditis. (A) Mannosylation is needed for uptake of the tracer in RAW 264.7 cell line. (B and C) The degree of RITC-MSA uptake is decreased by pretreatment of cold, unlabeled MSA in a dose-dependent manner. (D) Compared with the myocarditis only rats, the ⁶⁸Ga radiotracer signals in myocarditis rats pretreated with unlabeled MSA is decreased in the liver and the spleen, whereas the signal is significantly increased in other organs/tissues including the myocardium and the blood cavity. n=3 per group. SUV, mean standardized uptake value. (E) Autoradiography of the rat hearts demonstrate focally increased ⁶⁸Ga-NOTA-MSA uptake in the myocarditis rats (upper right panel), whereas the signal was diffusely increased in general in the myocarditis rats pretreated with unlabeled MSA (lower right panel). However, the degree of myocardial inflammation is similar between the two representative rats (upper and lower left panels). EAM, experimental autoimmune myocarditis.