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. 2016 Oct 17;84(4):671–687. doi: 10.3390/scipharm84040671

Table 5.

Results of robustness.

Variation Parameter Tailing Factor Theoretical Plates Area %RSD Resolution between S-isomer and LGP (R1) Resolution between LGP and MET (R2)
As per test method 1.3 4511 1.9 3.2 1.6
Flow (0.7 mL/min) 1.4 3698 1.5 3.1 1.5
Temperature: 23 °C 1.1 4675 4.3 3.4 1.0
Temperature: 33 °C 1.4 5280 3.3 2.9 2.0
Methanol: 360 mL 1.4 4467 1.9 2.5 2.7
Methanol: 440 mL 1.4 4696 2.5 3.4 1.2