Table 5.
Variation Parameter | Tailing Factor | Theoretical Plates | Area %RSD | Resolution between S-isomer and LGP (R1) | Resolution between LGP and MET (R2) |
---|---|---|---|---|---|
As per test method | 1.3 | 4511 | 1.9 | 3.2 | 1.6 |
Flow (0.7 mL/min) | 1.4 | 3698 | 1.5 | 3.1 | 1.5 |
Temperature: 23 °C | 1.1 | 4675 | 4.3 | 3.4 | 1.0 |
Temperature: 33 °C | 1.4 | 5280 | 3.3 | 2.9 | 2.0 |
Methanol: 360 mL | 1.4 | 4467 | 1.9 | 2.5 | 2.7 |
Methanol: 440 mL | 1.4 | 4696 | 2.5 | 3.4 | 1.2 |