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A Δhvo_2876 strain has fewer flagella or pili than a wild-type strain. Western blot analysis using anti-His antibodies was performed on protein extracts isolated from cell lysates (cell), and on TCA-precipitated proteins from the supernatants (sup) of wild-type, ΔaglB∆pilA1–6, or Δhvo_2876 liquid cultures expressing either (A) FlgA1His or (B) PilA2His. Molecular mass standards are indicated on the left (in kDa).
