Table 2.
Primers used for PCR amplification.
| Primer Name | Sequence (5’–3’) | Target Sequence |
|---|---|---|
| Fwko2876A | AAGCTAGGATCCAGAACCTTCCCCGGC | 700 bp upstream of hvo_2876 start codon |
| HVO2876KOXbaRv | GAACCTTCTAGAATCGGCATCGAGCCG | 700 bp downstream of hvo_2876 stop |
| codon Fwoverlap2876 | GACGAATCCACGCCTGCGTCGAACGCCGAG | 15 bp upstream of stop codon |
| Rvoverlap2876A | CTCGGCGTTCGACGCAGGCGTGGATTCGTC | 15 bp downstream of start codon |
| HVO2876CompFW | GATATCCATATGGCAACCTCGACG | At the start codon |
| HVO2876CompRV | GATATCGAATTCGGCGACGAGCTTGAAGTACAGC | At the stop codon |