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. 2017 Jan 1;26(1):28–43. doi: 10.1089/ars.2015.6615

FIG. 7.

FIG. 7.

The negative regulation of Nrf2 on NLRP3 inflammasome activation involved NQO1. tBHQ-pretreated WT BMDMs, Nrf2−/− BMDMs, and Nrf2 plasmid-transfected Nrf2−/− BMDMs were stimulated with LPS for 4 h and ATP for 1 h, and then, NLRP3, cleaved caspase-1, and IL-1β were detected by Western blot (A–C). IL-1β was detected by ELISA (D). WT BMDMs and Nrf2−/− BMDMs were treated with tBHQ for 12 h, and the mRNA levels of GCLM, GCLC, GST, HO-1, and NQO1 were measured by real-time RT-PCR (E, F). The expressions of the above mRNA were detected in THP-Ms treated with different stimulations (G). The results are representative of three independent experiments and are expressed as the mean ± SD. *p < 0.05, **p < 0.01, #p < 0.05, ##p < 0.01 compared with LPS + ATP, N.S means no significant difference.

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