Table 1.
Neuronal preparations and statistical tests used to evaluate DC-induced calcium relocation.
Type of experiment | Statistical test | Probability |
---|---|---|
(i) Axon in vivo | ||
Anodal versus cathodal | One-way ANOVA followed by Dunn's test | p < 0.001 |
Anode in versus control in | Mann–Whitney U Rank Sum test | p < 0.008 |
Cathode in versus control in | Mann–Whitney U Rank Sum test | p < 0.03 |
Cathode versus anode (combined segments) | Mann–Whitney U Sum Rank test | p < 0.001 |
(ii) Axon in vitro | ||
Inner and outer chambers versus “anode in” | Mann–Whitney U Sum Rank test | p < 0.036 |
Inner and outer segments combined versus control | Mann–Whitney U Sum Rank test | p < 0.001 |
(iii) Spinal cord in vitro | t-test | p < 0.029 |
(iv) Synaptosomes | ||
45Ca present during stim | ANOVA followed by Holm-Sidak | p < 0.001 |
45Ca added 6 hrs after stim | t-test | p < 0.003 |
45Ca in the presence of cobalt | t-test | p < 0.001 |
45Ca/neuroaminidase/stim versus no neuroaminidase/stim | t-test | p < 0.006 |
Na+ versus no Na+, no stim | ANOVA followed by Dunn's test | p < 0.001 |
Na+ versus no Na+, stim. | t-test | p < 0.029 |
Osmotic shock | t-test | p < 0.003 |
Different electrodes | Mann–Whitney U Sum Rank test | p < 0.001 |