Figure 12.

Blockade of TRPC channels by SKF96365 markedly improves podocyte and glomerular damage in response to DOCA treatment for 2 weeks in Podo GC-A KO mice. (A and B) Electron microscopy showed the normal appearance and only minimal widening of podocytes foot processes in Podo GC-A control mice after 2 weeks of DOCA treatment (upper panel of A and B). Massive foot process effacement was observed already 2 weeks after the start of DOCA treatment in Podo GC-A KO (A lower panel, B). These prominent changes were markedly improved by concomitant treatment with SKF96365 (A lower panel, B). C: capillary lumen; Bs: Bowman’s space. (C) PAS staining revealed glomerular hypertrophy of DOCA treated Podo GC-A KO mice (D). Moreover, DOCA induced a marked mesangial expansion in Podo GC-A KO which was significantly ameliorated by SKF96365 (E). Bar represents 50 µm. *P<0.05 versus sham or as indicated by bar; ^#P<0.001 versus sham or as indicated by bar.