Figure 8.

Fer-1, but not zVAD, prevents renal injury in FA-induced AKI in RIPK3-KO mice. (A) RIPK3-KO mice were pretreated with Fer-1 (n=7) or vehicle (n=8) 30 minutes before inducing AKI by an FA overdose and euthanized 48 hours later. Renal function was assessed by plasma creatinine and BUN levels, and cell death was assessed by TUNEL staining. Quantification is expressed as mean±SEM of seven or eight mice per group. **P<0.002 versus control; ^#P<0.05 versus AKI alone. (B) M30 cytodeath staining to assess caspase activation in RIPK3-KO mice during FA-induced AKI. Representative image and quantification as mean±SEM of seven mice per group. WT, wild type. Original magnification, ×200. Scale bars, 50 μm. **P<0.002 versus WT mice. (C) RIPK3-KO mice were pretreated with zVAD (n=8) or vehicle (n=8) 30 minutes before AKI induction by an FA overdose and euthanized at 48 hours. Renal function was assessed by plasma creatinine and BUN levels, and cell death was assessed by TUNEL staining. Quantification as mean±SEM of eight mice per group. *P<0.02 versus control; **P<0.002 versus control.