TABLE 1.
Plasmids used in this study
| Name | Insert |
|---|---|
| pNS3 | Truncated Rv3134c-dosR-dosS from H37Rv in pMV306-Kan; 507C plus 601C allele |
| pNS5 | Rv3134c promoter-Rv3134c-dosR from H37Rv in pMV306-Kan; 507C plus 601C allele |
| pNS6 | Rv3134c promoter-Rv3134c-dosR-dosS from H37Rv in pMV306-Kan; 507C plus 601C allele |
| pDC4 | Truncated Rv3134c-dosR-dosS from HN878 in pMV306-Kan; 507G plus 601T allele |
| pDC5 | Rv3134c promoter-Rv3134c-dosR from HN878 in pMV306-Kan; 507G plus 601T allele |
| pDC6 | Rv3134c promoter-Rv3134c-dosR-dosS from HN878 in pMV306-Kan; 507G plus 601T allele |
| pAA3 | Rv3134c promoter-Rv3134c-dosR-dosS from HN878 in pMV306-Kan; 507C plus 601T hybrid allele |
| pAA6 | Rv3134c promoter-Rv3134c-dosR-dosS from HN878 in pMV306-Kan; 507G plus 601T, DosR (D54E) |
| pAR2 | Rv3134c promoter-Rv3134c-dosS from HN878 in pMV306-Kan; 507G plus 601T allele (no dosR) |
| pdosT-wt | dosT from H37Rv in pMV361-Gent |
| pdosT-mut | dosT from HN878 (G775 frameshift mutation) in pMV361-Gent |
| pN-FLAGdosT-wt | 5′ FLAG-wild-type dosT in pMV361-Kan |
| pC-FLAGdosT-wt | Wild-type dosT-3′ FLAG in pMV361-Kan |
| pN-FLAGdosT-mut | 5′ FLAG-mutant dosT (G775 frameshift mutation) in pMV361-Kan |
| pC-FLAGdosT-mut | Mutant dosT (G775 frameshift mutation)-3′ FLAG in pMV361-Kan |
| pPR23ΔdosR+S | dosR-kan-dosS in pPR23; used for generating M. tuberculosis dosR-dosS KO mutants |
| pPR23ΔdosT/Hyg | dosT-hyg-dosT in pPR23; used for generating M. tuberculosis dosT KO mutants |
| pΔdosSv2.0 | dosS-kan-dosS in pPR23; used for generating M. tuberculosis dosS KO mutants |
| pPDM15 | dosR-hyg-dosR in pPR23; used for generating M. tuberculosis dosR KO mutants |
| pPDM4 | tgs1-hyg-tgs1 in pPR23; used for generating M. tuberculosis tgs1 KO mutants |