TABLE 1.
Strains, plasmids, and primers
| Strain, plasmid, or primer | Description or sequencea | Reference or source |
|---|---|---|
| Species and strains | ||
| E. coli DH5α | Cloning strain | 79 |
| S. aureus | ||
| RN4220 | Restriction-deficient transformation recipient | Lab Stocks |
| USA300 HOU | MRSA isolate cured of pUSA300-HOU-MRSA | 72 |
| HKM850 | USA300 HOU codY mutant; codY::tet | 75 |
| SH1000 | 8325-4 derivate with functional rsbU; rsbU+ | 80 |
| MJH502 | SH1000 sigB mutant; sigB::tet | 80 |
| BM2393 | USA300 HOU ΔrpoZ mutant | This study |
| AW2394 | USA300 HOU ΔrpoZ/pMK4::rpoZ rpoZ+ | This study |
| Plasmids | ||
| pMK4 | Gram-positive shuttle vector | 81 |
| pJB38 | Counterselectable plasmid to create silent mutations in S. aureus | 63 |
| Primers | ||
| OL2889 | ATGGAGCTCGCCATAATTTATCTTCCACCTTC | This study |
| OL2890 | ATGACGCGTGCAGTTGTTGCAATTAAATAC | This study |
| OL2891 | ATGACGCGTGTTTCGACCATTAAAAATATGTG | This study |
| OL2892 | ATGGGTACCCATTTCTTCAGCACTTTGAAC | This study |
| OL2991 | GCGTGAAATTGATGAACAAC | This study |
| OL2992 | CAGCAATTTCTTCTAACGCTC | This study |
| OL3008 | AGAAGATTAGCTTAGAGAGGTC | This study |
| OL3009 | GAAATGCTAATGGTGTCACA | This study |
| OL3377 | CGTCAGCAATTTCTTCTAACGCTCTACCAAC | This study |
| OL3753 | ATGGGATCCATCGCCAATTTCATCTTT | This study |
| OL3807 | ATGGGATCCATCTCCCTTAAATATCACTATG | This study |
| OL2393 | TCGTATGTTGTGTGGAATTG | This study |
| OL2394 | GTGCTGCAAGGCGATTAAG | This study |
| OL1297 | GTGGTGGCGTTTGTGC | This study |
| OL1298 | CCGACAGCAAACACACCCAT | This study |
| OL2129 | TAACATTCTCTGATGAAGTTG | This study |
| OL2130 | TTAAGTCTACAGCAGCTTG | This study |
| OL3136 | TCAATATTATCGGTGGATTTA | This study |
| OL3137 | GCAAATTATGATGTTGAAATAG | This study |
| OL3747 | CGTAGATGCAAATTATTACG | This study |
| OL3748 | CGTTAATGAAACAATTGGAC | This study |
| OL4099 | GGTTCACGTTCCTTTATC | This study |
| OL4100 | CAGGTTTACCATCTTTAGG | This study |
| OL4109 | GTTAAATGGTTTAATGCAGAA | This study |
| OL4110 | GTATCCATCTTCAGCGA | This study |
a
MRSA, methicillin-resistant S. aureus. Underlining denotes restriction sites.