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Effect of chloride ions concentration on (A) angiotensin-converting enzyme (ACE) and (B) Tityus serrulatus venom. For both, Abz-FRK(Dnp)P-OH hydrolysis was determined in Tris 100 mM, ZnCl2 10 µM buffer, with four different concentrations of NaCl: 0, 10 mM, 20 mM, and 50 mM. The result represents the mean of two independent experiments. The SD of kinetic results in each case was never greater than 5% of the value obtained.
