Table 1.
Hydrolysis of biologically active peptides by Tityus serrulatus venom and released fragments.
| Peptide | Fragment Sequence | Fragment Identification | MW | Venom Specific Activity (µM/µg/min) |
|---|---|---|---|---|
| Angiotensin I | DRVY | Ang(1–4) | 551.2 | 0.050 |
| IHPFHL | Ang(5–10) | 762.4 | ||
| HPFHL | Ang(6–10) | 649.3 | ||
| DRVYIHP | Ang(1–7) | 898.4 | ||
| DRVYIHPF | Ang II | 1045.4 | ||
| RVYIHPF | Ang III | 930.5 | ||
| FHL | Ang(8–10) | 415.2 | ||
| Bradykinin | RPPGF | BK(1–5) | 572.3 | 0.045 |
| RPPGFSP | BK(1–7) | 756.3 | ||
| PGFSPFR | BK(3–9) | 806.4 | ||
| Hemopressin | PVNFKFL | Hemo(1–7) | 863.4 | 0.400 |
| PVNFKF | Hemo(1–6) | 750.4 | ||
| PVNFK | Hemo(1–5) | 603.3 | ||
| KFLSH | Hemo(5–9) | 630.35 | ||
| FLSH | Hemo(6–9) | 502.25 |
Assays were carried out in 100 mM Tris buffer containing 50 mM NaCl, 10 µM ZnCl2, pH 7.0, at 37 °C, in a final volume of 100 µL. The results are shown as the mean of three independent experiments. The SD of kinetic was never greater than 5% of the value obtained.