Figure 2.

(a) Assay validation. Relative fluorescence of ∆der1, ∆hrd1, ∆yos9 and ∆nup120 knock-out mutant spheroplasts is indicated. Measurements were performed in the presence of RTA (5 µM) under induced culture conditions (3% galactose) over 20 h. Shown fluorescence values at 20 h were normalized to the fluorescence of spheroplasts treated with the negative control sample; (b) Impact of the indicated yeast deletion mutants on RTA trafficking. Fluorescence values of 2 × 10⁷ yeast spheroplasts of each mutant were displayed after 20 h induction (3% galactose) in the presence of RTA (5 µM). Values shown in (a,b) were normalized to the fluorescence of negative control spheroplasts, mean values and standard deviations are indicated. All measurements were at least repeated 3 to 12 times as independent experiments without technical replicates. The dotted line in (a) and (b) indicates the chosen threshold of 75% for a positive hit.