Figure 2.

Kinetics and limit of detection (LOD) of the SSA probes. A) Time dependence of fluorescent response of SSA^r and RNA analytes (left) and SSA^d and DNA analytes (right). The apparent difference in signal-to-noise ratio in comparison with Figure 1 B and C is due to the different analyte concentrations. Reaction mixtures contained: 1 µM DFHBI, 2.6 µM SSA^r_m and 3.6 µM SSA^r_f, 275 nM RNA analytes; or 2 µM DFHBI, 2 µM SSA^d_m and 3.6 µM SSA^d_f, 275 nM DNA analytes. B) Limits of detection (LOD) for SSA^r and SSA^d after 30 min of incubation. Averaged data from three independent experiments with standard deviations are presented.