Figure 3.

Selectivity of the SSA probes. A) Fluorescence response of SSA_r in the presence of 100 nM of either matched (A^r_m) or single base mismatched (A^r_mm) RNA analytes. B) Fluorescence response of SSA^d in the presence of 100 nM of either matched (A^d_m) or single base mismatched (A^d_mm) DNA analytes. The data are average values of 3 independent experiments with standard deviations. C) Photograph of the SSA^d samples from panel B upon excitation with transilluminator. The controls samples were as follows: DFHBI, DFHBI dye only; SAA, DFHBI dye, SSA_m, and SSA_f (no analyte). The concentrations were as specified in Figure 2 legend.