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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biomaterials. 2016 Sep 5;110:45–59. doi: 10.1016/j.biomaterials.2016.09.003

Fig. 4.

Fig. 4

(A) Schematic representation showing the assembly of the encapsulated HUVECs inside the bioprinted microfibers into a confluent layer of endothelium. (B) Confocal fluorescence micrograph showing the cross-sectional view of a three-layer scaffold at Day 14, indicating the formation of the endothelium by the HUVECs. (C) High-resolution confocal fluorescence micrograph showing the distribution of the HUVECs in a single microfiber at Day 14. Left: projection view; Right: 3D rendering of the tubular structure at the position of the dotted line. (D) Confocal fluorescence micrograph showing the GFP-HUVECs in a single fiber for CD31, GFP, and nuclei. (E, F) Fluorescence micrographs showing the distribution and spreading of GFP-HUVECs in the bioprinted microfibrous scaffolds with different aspect ratios of unit grids at Day 1 and Day 15, respectively.