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. 2016 Sep 30;3(4):ofw196. doi: 10.1093/ofid/ofw196

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Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 3. — Sensitized Clostridium difficile spores are killed by low pH. Six log₁₀ colony-forming units (CFUs) of C difficile (American Type Culture Collection [ATCC] 43598) were inoculated into 1mL water, quaternary ammonium, germinants, or quaternary ammonium containing germinants. Spore suspensions were incubated under ambient conditions for 30 minutes, split into 2 sets, and then washed 3 times with water. The treated spores were exposed to pH 2.0 water or neutral pH water for 10 minutes. Log₁₀ CFU reduction was determined by comparing recovery of spores treated with neutral pH water and recovery of spores treated with pH 2.0 water. The means of data from triplicate experiments are presented. Error bars indicate standard error.