Fig. 3.

Cell proliferative effect of yokukansan on B65 neuroblastoma cells. (A) Images of B65 neuroblastoma cells cultured with and without 500 μg/mL yokukansan. Pictures were taken after treatment with 500 μg/mL yokukansan for 48hr. The scale bars represent 500 μm. (B) WST-8 cell proliferation assay of B65 neuroblastoma cells cultured with or without various concentrations of yokukansan after treatment for 24, 48 and 72 h. The vertical axis shows the proliferation rate relative to that in the control without yokukansan. The histogram shows means ± S.D (n = 3). When the absolute absorbance value was statistically different from control without yokukansan, it was considered to be statistically significant (*; p < 0.05 vs. control absolute absorbance value).