Fig. 1.

Hibiscus extract inhibits melanoma cell growth. (a) B16F10 melanoma cells and nontransformed NIH3T3 fibroblasts (5,000 cells per well) were grown overnight before being incubated for 72 h with indicated concentrations of hibiscus extract and photographed (bar = 280 microns). (b) Cells were counted and shown as the number of cells in a 500 × 500 micron area in the center of each well (mean + SEM, n = 3). Single and double asterisks denote p < 0.05 and p < 0.01 compared to untreated control cells, respectively.