Fig. 2.

Hibiscus extract contains multiple bioactive components. (a)B16F10 melanoma cells (5,000 cells per well) were grown overnight before being incubated for 72 h with no extract (Control), unfractionated hibiscus extract (Total), or fractions containing components of hibiscus extract greater than 50 kD (>50 kD), between 3 and 50 kD (3–50 kD), or less than 3 kD (<3 kD) and photographed (bar = 350 microns). (b) Cells were counted and shown as percent of controls (mean + SEM, n = 3). Single and double asterisks denote p < 0.05 compared to control cells treated without extract and p < 0.1 between cells treated with total extract or fractions with components less than 3 kD as indicated.