Fig. 3.

Hibiscus extract inhibits Src transformed cell growth, but not oncogenic Src kinase activity. (a) LA25 cells were grown overnight at nonpermissive temperature (40 °C) before being incubated for 24 h at permissive (33 °C) and nonpermissive (40 °C) with concentrations of hibiscus extract as indicated. Cells were then stained with Trypan blue, and examined to measure cytotoxicity. Data are shown as the percent of live cells (mean + SEM, n = 3). (b) Total (v-Src) and active (p-Src) were detected by Western blotting from cells grown at nonpermissive temperature (Src off) and permissive temperature (Src on) with and without 4 mg/ml hibiscus extract as indicated.