Subject area	Biology
More specific subject area	2-deoxy-d-glucose treatment, O-GlcNAcylation of cellular proteins
Type of data	Western blotting
How data was acquired	Western blotting using a chemiluminescent substrate (ECL Prime Western Blotting Detection Reagent, GE Healthcare UK Ltd.) and an image analyzer (Light-Capture ATTO Co.).
Data format	Raw data for Western blotting
Experimental factors	Cells were treated with 2-deoxy-d-glucose (2DG) and cellular proteins were analyzed by ECL Prime Western Blotting system.
Experimental features	Human teratocarcinoma NCCIT cells were incubated with a culture medium supplemented with 10 mM 2DG for 24–168 h. Protein extracts of the cells and the immunoprecipitates of anti-Sp1 antibody (D4C3) were subjected to Western blotting.
Data source location	Bioproduction research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba
Data accessibility	The data are provided in this article.