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. 2016 Dec 18;11:275–285. doi: 10.1016/j.redox.2016.12.017

Fig. 2.

Fig. 2

One-step purification of VC1-His-Strep from the culture supernatant ofP. pastorisrecombinant cells. (A) Culture supernatant was collected at 72 h after ethanol/methanol induction of KM71-VC1-His-Strep cells and an aliquot (30 μl) was directly analyzed by SDS-PAGE. (B) Secreted VC1-His-Strep (2 μg) was purified from the culture supernatant by Nikel-affinity chromatography. Proteins were separated by SDS-PAGE and stained by Coomassie blue. Arrows indicate the two glycoforms of VC1-His-Strep.