Fig. 6.

Time course analysis of the dose-dependent modification of HSA by MDA. Recombinant HSA was incubated with increasing concentrations of MDA as explained in Materials and methods. At 24, 48 and 72 h of incubation, aliquots were withdrawn and after removal of free MDA, pull-down assays with the VC1-resin or control (CTRL)-resin were performed as explained in Fig. 3. A specific binding of both the monomer of modified HSA and of the HMW-species to the VC1-resin was detected at increasing concentrations or time of incubation.