Table 2.

AGE-modified peptides identified by MS analysis in high MW-species BSA-ribose captured by VC1-resin.

Modification	ΔM	Peptide sequencea	Amino acidb	Charge	MH+c	ppm
Carboxymethyl	+58.00548	R*HPEYAVSVLLR	R360	+3	1497.81552	−1.18
Pyrraline-derived	+78.01056	K*VPQVSTPTLVEVSR	K437	+3	1717.94822	−0.10
		ALK*AWSVAR	K235	+3	1079.59861	−1.01
		FPK*AEFVEVTK	K248	+3	1372.71704	+1.64
Schiff base	+132.04426	K*VPQVSTPTLVEVSR	K437	+3	1771.98740	+3.00
		ALK*AWSVAR	K235	+3	1133.62992	−3.06
		R*HPEYAVSVLLR	R360	+3	1571.85331	−1.76
Unknown	+218.07903	K*VPQVSTPTLVEVSR	K437	+3	1858.01419	−1.43
		K*QTALVELLK	K548	+3	1360.79386	+0.32
		R*HPEYAVSVLLR	R360	+3	1657.89438	+2.14
Methyl imidazolone	+54.01056	YLYEIAR*R	R167	+3	1137.60450	−0.57
Carboxyethyl	+72.02113	K*VPQVSTPTLVEVSR	K437	+3	1711.95627	−1.57
		FK*DLGEEHFK	K36	+3	1321.64435	+1.51

The high MW species of BSA-ribose (≥250 kDa) shown in Fig. 3B were subjected to in-gel digestion.

^aThe K* or R* indicate the position of the modified residue.

^bThe numbering refers to the amino acid residue in the sequence of the precursor BSA that includes the signal peptide.

^cExperimentally determined monoisotopic mass.