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. 2016 Dec 18;107(12):1721–1729. doi: 10.1111/cas.13084

Figure 4.

Figure 4

B6 mice were immunized once a week for 2 weeks with OVA 257–264 peptide (OVA) (five mice/group). One week after the last immunization, spleen cells were subjected to γ‐interferon (IFN‐γ) enzyme‐linked immunospot assay (ELISPOT). If the values were higher than the mean + 2SD of the number of IFN‐γ‐producing cells in mice immunized with OVA 257–264 alone (8.58/106 spleen cells), the response was considered to be augmented. Representative ELISPOT images of negative and positive cases are shown. Data are representative of two experiments. *P < 0.05 by Fisher's exact test. GR, glycyrrhizin; IMQ, imiquimod.