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. 2016 Dec 18;107(12):1721–1729. doi: 10.1111/cas.13084

Figure 6.

Figure 6

Message levels of various immune‐related genes in mice immunized with OVA 257–264 peptide (OVA), measured at the inoculation skin site (a) and the spleen (b) 6 h (black column), 12 h (gray), and 24 h (white) after immunization, were analyzed by real‐time PCR. Samples of mRNA were extracted from the pooled tissues of three mice from each treatment group. The expression levels were calculated relative to GAPDH expression in untreated mouse skin or spleen. (b) Message levels of interleukin‐1β (IL‐1β; pro‐inflammatory cytokine), interferon‐α (IFN‐α; upregulator of CTL induction), and IL‐6 (pro‐inflammatory cytokine) in the spleen are shown. The expression profile of the other genes among the treatment groups in the spleen was similar to that of the skin (data not shown). Data are representative of two experiments. Arg1, arginase 1, mediator of immunosuppressive M2 macrophages and myeloid‐derived suppressor cells; COX‐2, responsible for inflammation mediators such as prostaglandin E2; GR, glycyrrhizin; IL‐10, immunosuppressive cytokine; IMQ, imiquimod; TNF‐α, tumor necrosis factor‐α, pro‐inflammatory cytokine.