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. Author manuscript; available in PMC: 2018 Feb 1.

Published in final edited form as: J Inorg Biochem. 2016 Nov 23;167:124–133. doi: 10.1016/j.jinorgbio.2016.11.027

Fig. 2 — A) UV-visible spectra of ferric (black —), ferrous ( ), and ferrous carbonyl ( ) complexes of HtaA-CR2. The ferric and ferrous carbonyl samples were in 50 mM Tris-HCl pH 7.0; the ferrous HtaA-CR2 was in 50 mM Tris-HCl pH 7.0. B) High-frequency rR spectra of a) ferric HtaA-CR2, 406.7 nm excitation, b) ferrous HtaA-CR2, 413.1 nm excitation, and c) ferrous-CO HtaA-CR2 complex, 413.1 nm excitation, under the same buffer conditions listed in A. Laser powers incident on the sample for a and b were 5 - 8 mW while c was acquired with 2.5 mW.

Inline graphic — A) UV-visible spectra of ferric (black —), ferrous ( ), and ferrous carbonyl ( ) complexes of HtaA-CR2. The ferric and ferrous carbonyl samples were in 50 mM Tris-HCl pH 7.0; the ferrous HtaA-CR2 was in 50 mM Tris-HCl pH 7.0. B) High-frequency rR spectra of a) ferric HtaA-CR2, 406.7 nm excitation, b) ferrous HtaA-CR2, 413.1 nm excitation, and c) ferrous-CO HtaA-CR2 complex, 413.1 nm excitation, under the same buffer conditions listed in A. Laser powers incident on the sample for a and b were 5 - 8 mW while c was acquired with 2.5 mW.