Table 2. ROC analysis for cytokines in discriminating TB infected vs. uninfected group.
| Stimulant | Cytokine | TBI (ATB+LTBI)a | CONa | AUC(95%CI) | P-value | Cut-off (pg/ml)b | Sensitivity% | Specificity% |
|---|---|---|---|---|---|---|---|---|
| 24h | ||||||||
| Unstimulated | IFN-γ | 0.7(0–3.1) | 1.5(0–4.6) | 0.5325(0.4449–0.6201) | 0.4667 | — | — | — |
| IP-10 | 180.7(97.3–340.9) | 150.4(82.4–318.5) | 0.5541(0.4679–0.6403) | 0.2255 | — | — | — | |
| IL-2 | 0.7(0–3.7) | 1.4(0–5.6) | 0.5298(0.4423–0.6172) | 0.5043 | — | — | — | |
| ESAT-6/CFP-10 | IFN-γ | 195.1(72.3–428.6) | 0.3(0–3.1)c | 0.9752(0.9507–0.9997) | <0.0001 | 18.5 | 94.4 | 100 |
| IP-10 | 3257.3(1409.2–4824.3) | 98.7(17.4–317.5)c | 0.9282(0.8911–0.9685) | <0.0001 | 1279.5 | 77.8 | 100 | |
| IL-2 | 169.0(51.6–451.6) | 2.1(0.0–7.8)c | 0.9298(0.8911–0.9685) | <0.0001 | 20.2 | 84.3 | 94.2 | |
| PPD | IFN-γ | 334.0(169.5–710.4) | 242.7(15.7–367.4)c | 0.6797(0.5995–0.7599)e | <0.0001 | 88.9 | 88.9 | 39.1 |
| IP-10 | 4106.2(1916.0–7898.7) | 1220.3(345.1–3123.2)c | 0.7560(0.6847–0.8273)e | <0.0001 | 1257.3 | 86.1 | 53.6 | |
| IL-2 | 584.6(303.9–1139.0) | 215.7(67.4–727.3)c | 0.6987(0.6163–0.7810)e | <0.0001 | 227.5 | 84.3 | 53.62 | |
| 72h | ||||||||
| Unstimulated | IFN-γ | 29.7(11.2–51.1) | 16.5(5.1–28.3)d | 0.6467(0.5674–0.7261) | 0.0014 | 30.7 | 48.2 | 79.7 |
| IP-10 | 238.6(106.6–447.5) | 129.6(68.9–216.3)c | 0.6809(0.6034–0.7584) | <0.0001 | 249.1 | 48.2 | 89.9 | |
| IL-2 | 17.4(2.3–54.3) | 9.4(0.0–25.6)d | 0.6027(0.5185–0.6807) | 0.0201 | 34.6 | 37.0 | 84.1 | |
| ESAT-6 /CFP-10 | IFN-γ | 648.7(243.1–1038.2) | 11.2(1.9–18.8)c | 0.9697(0.9410–0.9983) | <0.0001 | 58.4 | 94.4 | 100 |
| IP-10 | 4353.5(1772.3–6703.7) | 231.7(154.7–542.2)c | 0.9147(0.8717–0.9576) | <0.0001 | 1472.2 | 79.6 | 98.6 | |
| IL-2 | 275.9(109.7–625.4) | 7.5(2.9–14.9)c | 0.9616(0.9365–0.9867) | <0.0001 | 46.3 | 87.0 | 95.7 | |
| PPD | IFN-γ | 747.6(366.8–1143.2) | 356.8(228.1–537.5)c | 0.7052(0.6293–0.7811)e | <0.0001 | 551.8 | 59.3 | 78.3 |
| IP-10 | 4561.5(2288.3–8828.2) | 157.1(517.0–4454.2)c | 0.7050(0.6252–0.7848)e | <0.0001 | 1911.3 | 81.5 | 55.1 | |
| IL-2 | 681.9(364.3–1359.2) | 255.7(73.3–806.8)c | 0.7078(0.6283–0.7873)e | <0.0001 | 355.2 | 76.9 | 59.4 |
a Data are presented as median concentration in pg/ml (interquartile range).
b The cut-off values are determined at the maximum Youden’s index (YI), i.e. sensitivity+ specificity -1.
Statistical significance was determined versus TBI group,
c p<0.0001;
d 0.0001≤p<0.001. (Mann Whitney test)
Statistical significance was determined versus AUC stimulated by ESAT-6/CFP-10,
e p<0.0001.
AUC = Area under the ROC curve.