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. 2016 Dec 22;12(12):896. doi: 10.15252/msb.20167087

Figure 3. sigC single‐cell traces do not show a double peak, but show that sigC is negatively auto‐regulated.

Figure 3

  1. Neither single‐cell traces nor a population average of a reporter for sigC, PsigC‐YFP, shows a double peak of expression. 664 cells from nine movies (with up to 213 cells per time point) were collected.
  2. Measure of the distance between the first peak in each circadian cycle and the following peak confirms that there is only one peak per circadian cycle.
  3. The amplitude in sigC expression is raised by 3.5‐fold in a sigC knock‐out strain, consistent with a sigC‐negative auto‐regulation. 1,084 cells from six movies (with up to 352 cells per time point) were collected.
  4. Measurement of the distance between the first peak in each circadian cycle and the next peak confirms that there is only one peak per circadian cycle.
Data information: Pink and light blue shades represent one standard deviation from the mean. Black and green lines represent two single‐cell traces.Source data are available online for this figure.