Figure EV4. Double peaks in P_rpoD6‐YFP expression are dependent on sigC .

1. Measure of the distance between the first peak in each circadian cycle and the following peak shows only a subfraction of cells display a double peak. 947 cells from 12 movies (with up to 272 cells per time point) were collected.
2. Single‐cell lineages of a representative movie show that all lineages show either a second peak, or a shoulderlike feature, in P_rpoD6‐YFP levels.
3. In the sigC deletion mutant, the two‐peak oscillations are abolished. 1,352 cells from seven movies (with up to 502 cells per time point) were collected.
4. Single‐cell lineages of a representative movie show a single peak of P_rpoD6‐YFP.

Source data are available online for this figure.