Figure EV1. Ultrafast arsenic adaptation is unlikely to be driven by standing genetic variation.
As(III) adaptations were repeated, but starting from four separate founder populations, each derived from different (single) founder clones (P5–P8). The probability of standing variants being present at the onset of selection in all four populations is small (see Materials and Methods). Samples were extracted at the end of each batch cycle, that is every 5th generation, stored as a frozen fossil record, revived in fresh medium and cultivated in 5 mM As(III) at high replication (n = 10). Fitness components were extracted and analysed as for Fig 1B.Source data are available online for this figure.