Figure 3.

Hypoxia-dependent clonogenic inhibition by Benznidazole is independent of tumor cell types. Human tumor cell lines were incubated with Benznidazole (BZDZ, 100 µM) or DMSO at normoxia (20% O₂) or anoxia (0% O₂). (A) Images of colony growth for tumor cells plated at 3,000 cells/well in 6-well plates to demonstrate the synergistic effects of BZDZ and hypoxia. (B) Relative surviving fraction (mean ± SD, n = 3) was calculated using data from the plates seeded at the clonal density of 300 cells/well. The data from DMSO-treated cells at 20% O₂ were used as control. These results were validated by at least 2 independent experiments.