Figure 6.

Benznidazole treatment does not induce PARP1 cleavage. MDA-MB-231 breast cancer cells (A) and SK-N-ER neuroblastoma cells (B) were incubated for 24 hours with Benznidazole (abbreviated as B, 100 µM) or dimethyl sulfoxide (abbreviated as D) under aerobic (20% O₂) and anoxic (0% O₂) conditions, respectively. For the reoxygenation treatment, the hypoxia-treated cells were placed in the aerobic incubator for 24 hours. Etoposide (ETOP, 10 µM) was used as positive control. PARP1 cleavage was examined by Western blot analysis with β-actin as the internal loading control. These results were confirmed by more than 3 independent experiments.