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. 2016 Dec 21;11:322–334. doi: 10.1016/j.redox.2016.12.021

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4. — The induction of autophagy accelerates LD disappearance in quiescent HSCs. Freshly isolated HSCs were treated with control-Vector or Atg5-plasmid, and then were cultured for 5 days. (A) Western blot and densitometric analysis showing LC3-I/II levels. (B) ORO absorbance and retinol measurement showing the content of lipid droplets. (C) Nile red staining and Perilipin A immunostaining showing the changes of cellular lipid content. For the statistics of each panel in this figure, data are expressed as mean±SD (n=3); *P<0.05 versus control, **P<0.01 versus control, ***P<0.001 versus control.