Table 1.

Summary of Cryo-EM data.

DOI: http://dx.doi.org/10.7554/eLife.21829.002

Imaging
Microscope	Titan Krios I, 300keV (FEI)
Detector	K2 Summit direct electron detector (Gatan)
Energy filter	10 eV (Gatan)
Data collection
Pixel size	1.04 Å
Movies	3875
Frames	50
Total exposure time	10 s
Exposure time per frame	0.2 s
Total exposure	74 electrons/Å2
Exposure per frame	1.48 electrons/Å2/frame
Defocus range	−1.5 to −3.5 μm
Final reconstruction
Number of particles	501,973
B-factor correction	−150 Å2