Table 1.
Overall screening analyses that showed an alteration of miRNA-34a expression after ionizing radiation.
| Species | Cell type | Cell lines | Irradiation dose (Gy) |
Collection time after radiation |
Screening method |
MiR-34a expression | Ref. |
|---|---|---|---|---|---|---|---|
| Human | Thyroid | Primary cells from biopsies |
1 & 10 | 4 & 24h | Microarray and qRT- PCR |
↑ at 4h after 1 & 10Gy ↓ at 24h after 1 & 10Gy |
(Nikiforova et al., 2011) |
| Human | Prostate | LNCaP PC3 |
1×5 & 1×10 10×0.5 & 10×1 |
24h after last dose |
Microarray and qRT- PCR |
↑ after 10×0.5 & 1Gy in LNCap ↑ after 10×1Gy in PC3 |
(John-Aryankalayil et al., 2012) |
| Human | Lung | A549 | 40 | 24h | Microarray | ↑ | (Shin et al., 2009) |
| Human | Lymphoblast | IM9 | 10 | 24h | Microarray | ↑ | (Cha et al., 2009) |
| Human | Coronary artery |
HCAEC | 1×10 & 5×2 | 6 & 24h after last dose |
Microarray | ↑ at 24h after 1×10Gy ↑ at 6 & 24h after 5×2Gy |
(Palayoor et al., 2014) |
| Human | Glioblastoma | A172 | 2×15 & 2×30 |
- | qRT-PCR | ↑ after 2×30Gy ↓ after 1×15Gy |
(Sasaki et al., 2012) |
| Human | Lymphoblast | TK6 | 2 | 4, 8, 12 & 24h | Sequencing | ↑ at 8 & 24h ↓ at 4 & 12h |
(Chaudhry et al., 2013) |
| Human | Lymphocyte | Whole blood |
0.2 & 2 | 4 & 24h | Microarray and qRT- PCR |
↑ at 24h after 0.2 & 2Gy ↓ at 4h after 0.2Gy |
(Girardi et al., 2012) |
| Human | T- lymphocyte |
Whole blood |
1, 2, 3, 4 & 5 |
15 & 30min, 1, 1.5, 2, 4, 5, 6 & 24 |
qRT-PCR | ↑ at 24h after 1, 2, 3, 4 & 5Gy ↑ at 3, 4, 5, 6 & 24h after 2Gy |
(Kabacik et al., 2015) |
| Mouse | Liver | Kunming | 4 (WBI) | 14 days | Sequencing and qRT- PCR |
↑ | (Lu et al., 2016) |
| Mouse | Skin Muscle Subcutaneous tissue |
CH3 | 35 (right hind leg) |
7, 14, 50, 90 & 120 days |
Microarray and qRT- PCR |
↑ at 7, 14 & 120 days | (Simone et al., 2014) |
| Mouse | Spleen | C57BL/6 | 2.5 (WBI) | 6h | Microarray and qRT- PCR |
↑ | (Ilnytskyy et al., 2008) |
| Rat | Retinal ganglion |
RGC-5 cells | 6 | 5 days | Microarray and qRT- PCR |
↑ | (Wang et al., 2015) |
| Rat | Lung | Wistar | 20 (right lung) |
12 weeks | Microarray | ↑ | (Xie et al., 2014) |
| Rat | Mammary gland |
Long-Evans | 0.1 & 2.5 (WBI) |
6 & 96h 4 & 24 weeks |
Microarray and qRT- PCR |
↑ at 96h & 24 weeks after 0.1 & 2.5Gy |
(Wang et al., 2013) |
| Rat | Mammary gland |
Long-Evans | 0.1 & 2.5 (WBI) |
96h | Microarray | ↑ after 0.1 & 2.5Gy | (Luzhna and Kovalchuk, 2014) |
WBI: Whole-Body Irradiation